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To obtain ratiometric images, the masks were applied to the background-subtracted images and the FP channel was divided by the SiR channel in a pixel-by-pixel manner within the ROIs

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Both values fall within the acceptable recovery range of 80120% 36

Understanding these individual differences is crucial for developing targeted interventions aimed at mitigating the effects of neuroinflammation in those affected by chronic alcohol use

Aminoacridine, abemaciclib, and proflavine notably increased expression levels of RSPO3 and RSPO4 genes, with aminoacridine notably enhancing RSPO3 levels by 19-fold, the highest among tested drugs